Interactions with Milkweed
Research Projects
Monarch larvae are specialist herbivores of plants in the family Asclepiadaceae
(milkweeds), and have been recorded feeding on 27 different North American species
in this family (Malcolm and Brower 1989). The larvae sequester toxic steroids, known
as cardenolides, from milkweed (Brower 1969; Brower
& Glazier 1975; Malcolm 1991, 1995), and they use these cardenolides as a defense
against predators. The bad taste and toxicity of both the larvae and adults are
advertised by conspicuous, warning coloration. When a bird predator tastes a monarch,
it learns to associate this color pattern with the bad taste, and avoids preying
on monarchs in the future.
Much of the research on the relationship between monarchs and milkweed has focused
on the following questions:
- How do chemicals in milkweed benefit monarchs?
- How do different milkweeds and monarchs vary in the type and concentration of
cardenolides that they contain?
- How do milkweed defenses affect monarch larvae?
How Do Chemicals in Milkweed Benefit Monarchs?
Early in the 20th century, Poulton (1909) suggested that the reason that monarchs
feed only on milkweeds might be because chemicals in the milkweeds provide them
with some protection from predators. However, it was not until the 1960’s
that researchers discovered that cardenolides were the chemicals in milkweed that
made monarchs toxic and bitter-tasting (Parsons 1965). Since then, several studies
have shown that monarchs are able to sequester these toxic compounds. For example,
Figure 1, drawn from data collected by Malcolm and Brower (1989) shows the range
of cardenolide concentrations found in one species of milkweed, A. viridis,
and adult monarchs that fed on that milkweed as larvae. Since the adults do not
eat milkweed, this shows that they have stored the cardenolides that they ingested
as larvae.
The cardenolides obtained from milkweed make monarchs toxic to many vertebrate predators
(e.g. Brower et al. 1974, Rothschild et al. 1975). For example, captive bluejays
fed monarchs containing cardenolides throw up after eating the monarchs, and the
probability of their throwing up increases with increasing cardenolide concentration
in the monarch (e.g. Brower 1969, Brower et al. 1972, Brower and Glazier 1975).
Other work has shown that wild monarchs containing high levels of cardenolides are
also less susceptible to natural predation by both birds (Fink and Brower 1981)
and mice (Glendinning et al. 1988).
Protection from vertebrate predators is probably most important to later larval
instars, pupae, and adults, whereas earlier instars are more likely to be eaten
by arthropods such as mites, spiders, ants, wasps and bugs (Figure 2). Vertebrates
tend to be long-lived and able to use learning to avoid noxious foods, and most
people agree that the bright colors found on monarchs and other toxic insects help
to identify them to vertebrate predators that have learned to associate these colors
with bad tastes (e.g. Turner 1984). However, it is not clear that invertebrate predators
learn to associate bright colors with bad taste, or even that monarchs are toxic
to invertebrate predators. Since most mortality of monarchs probably occurs in the
early instars (see Monitoring data),
the lack of knowledge on the relationship between milkweed, monarchs and invertebrate
predators represents an important gap in our knowledge.
Variation Among Milkweeds and Monarchs in Toxin Concentration
Roeske et al. (1976) showed that different species of milkweed contained different
concentrations of toxins, and that monarchs reared on these species also varied
in the amount of toxins in their bodies. However, there is not a perfect correlation
between the amount of cardenolides in plants, and the monarchs that consume them.
Figure 3 (below) shows the concentration of cardenolides in various milkweed species,
and the concentrations in adult monarchs that were fed these milkweeds as larvae.
Monarchs that were fed A. viridis from Florida had the highest cardenolide
concentrations, even though the plants themselves had intermediate levels (see figure
3). It appears that monarchs fed high cardenolide plants do not concentrate the
toxins as effectively as do monarchs from intermediate and low cardenolide plants
(Malcolm and Brower 1989).
In addition to variation in cardenolide concentration between milkweed plants of
different species, there is also a great deal of variation within plant species.
For example, one of the plant species shown in figure 3, A. syriaca (common
milkweed), had cardenolide concentrations ranging from 0 to 792 µg/0.1 g dry
weight (Malcolm and Brower 1989). While it is not completely clear what causes so
much variation, recent work by Steve Malcolm and his collaborators (e.g. Malcolm
and Zalucki 1996) showed that plants produced more cardenolides within 24 hours
after being damaged. This suggests that cardenolides are an example of an "inducible"
plant defense; this would benefit the plant, since it would only have to invest
in defenses when it needed them. Perhaps the variation in plant cardenolide concentration
is due partly to variation in damage by herbivores.
Recent work by Alfonso Alonso has shown that monarchs lose cardenolides as they
get older. Over a period of 30 days the concentration of cardenolides in the wings
and abdomens of monarchs kept in an outdoor cage decreased by about 600 µg/0.1
g dry weight (Alonso-Mejia and Brower 1994). Similar decreases in concentration
occur over the overwintering period in Mexico (Alonso 1996), suggesting that monarchs
become less protected from predators as they get older. Alonso thinks that this
decrease is due to scale loss from the butterflies’ wings, denaturation of
the cardenolides, and excretion (Alonso 1996).
Effects of Milkweed Defenses on Monarch Larvae
Monarchs are thought by many people to be specialists that incur little cost to
feeding on milkweeds. In fact, they do appear to benefit both nutritionally and
defensively from their milkweed diet (Malcolm 1991, 1995). However, plants use a
variety of defenses against the animals that eat them, and the cardenolides in milkweeds
are present to protect the plants, not to protect the monarchs eating them. The
defensive system of milkweeds was well-described by Dussourd (1993): "milkweed
leaves contain a ramifying network of latex canals pressurized with a lethal brew
of toxic cardenolides in a quick-setting glue." Thus, it should not
be surprising that monarchs do suffer some ill effects from feeding on milkweed.
Recent evidence has shown that the larvae are negatively affected by the milky latex
characteristic of milkweeds, which can gum up the mandibles of small larvae so that
they can no longer eat. Zalucki & Brower (1992) conducted an experiment in which
they followed almost 700 monarchs from the egg stage to the second instar stage.
They found very low survival (from 3 to 11%), and determined that about 30% of larvae
were killed when they became mired in milkweed latex. Supporting this result, Malcolm
and Zalucki (1996) and Zalucki & Malcolm (1998) found higher survival of first
instar larvae when they fed on leaves on which the latex flow had been cut off.
In addition to the negative effects of the latex, there is evidence that monarchs
may also be affected negatively by milkweed cardenolides (Zalucki et al. 1990).
Early instar survival is lower on plants with high cardenolide levels, and larvae
that happened to swallow some of the latex had a strong response, as described by
Zalucki & Brower (1992): "the anterior portion of the larva’s body
looked pale, the larva backed onto the silk mat, assumed a position with its prolegs
anchored, and raised the anterior part of its body and tucked its head down. The
larva would remain cataleptic in this position for up to 10 minutes before continuing
its biting behavior." Other evidence that high cardenolide levels may
be harmful to larvae is the fact that females prefer to oviposit on plants that
have intermediate levels of this toxin (Zalucki et al. 1990, Oyeyele and Zalucki
1990, Van Hook and Zalucki 1991).
Monarch larvae appear to use a variety of strategies to avoid these affects. Small
larvae use a 'trenching' behavior, in which they chew a small circle through the
surface of the leaf, making a circular area to which latex does not flow (figure
4). Larger larvae cut through the mid-vein of a leaf, cutting off latex flow to
the entire leaf (figure 5) Both of these behaviors provide protection from the sticky
latex, and possibly also from the toxins, which are more concentrated in the latex
(Zalucki and Brower 1992).
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References
- Alonso-Mejia, A. and L.P Brower. 1994. From model to mimic: age dependent unpalatability
in monarch butterflies. Experientia 50:176-181
- Alonso-Mejia, A. 1996. Biology and conservation of overwintering monarch butterflies
in Mexico. Ph.D. thesis, University of Florida.
- Brower, L.P. 1969. Ecological chemistry. Scientific American 220:22-29.
- Brower, L.P. 1984. Chemical defense in butterflies. Pages 109-134 in R. Vane-Wright
and P. Ackery (editors), The biology of butterflies. Academic Press.
- Brower, L.P., & S.C. Glazier. 1975. Localization of heart poisons in the monarch
butterfly. Science 188:19-24.
- Brower, L.P., P.B. McEvoy, K.L. Williamson and M.A. Flannery. 1972. Variation
in cardiac glycoside content of monarch butterflies from natural populations in
eastern North America. Science 177:426-429.
- Brower, L.P. and C.M. Moffit. 1974. Palatability dynamics of cardenolides in the
monarch butterfly. Nature. 249:280-283.
- Dussourd, D.E. 1993. Foraging with finesse: Caterpillar adaptations for circumventing
plant defenses. Pages 92-131, in, N.E. Stamp & T.M. Casey (editors),
Caterpillars. Ecological and Evolutionary Constraints on Foraging. New York:
Chapman & Hall.
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butterflies in Mexico. Nature 291:67-70.
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and their comparative processing by monarch butterflies. Rec. Adv. Phytochem.
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